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Lack of influence of test meal fatty acid composition on the contribution of intestinally-derived lipoproteins to postprandial lipaemia

Published online by Cambridge University Press:  09 March 2007

Kim G. Jackson ,
Antonios Zampelas ,
Jacky M. E. Knapper ,
Caroline C. Culverwell ,
John Wright ,
Barry J. Gould  and
Christine M. Williams
Kim G. Jackson*
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK
Antonios Zampelas
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK
Jacky M. E. Knapper
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK
Caroline C. Culverwell
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK
John Wright
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK
Barry J. Gould
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK
Christine M. Williams
Affiliation:
Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford GU2 5XH, UK
*
*Corresponding author: fax +44 (0)118 931 0080, email k.jackson@afnovell.reading.ac.uk
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Abstract

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The extent and duration of postprandial lipaemia have been linked to risk of CHD but the influence of dietary variables on, and the relative contributions of, exogenous (chylomicron) and endogenous (VLDL) triacylglycerols to the total lipaemic response have not been comprehensively evaluated. In the present study the triacylglycerol, apolipoprotein (apo) B-48 and retinyl ester (RE) responses to three test meals of varying monounsaturated (MUFA) and saturated fatty acid (SFA) content were measured in the triacylglycerol-rich lipoprotein (TRL) fraction of plasma (ρ = 1·006 g/ml) for 9 h after meal consumption. Fifteen healthy normolipidaemic young men consumed, on separate occasions, three test meals which were identical apart from their MUFA and SFA contents. Expressed as a percentage of total energy the MUFA/SFA contents of the meals were: (1) 12 %/17 %; (2) 17 %/12 % and (3) 24 %/5 %. The contribution of the intestinally-derived lipoproteins (chylomicrons) to the lipaemic response was investigated by determining the time to reach peak concentration and the total and incremental areas under the time response curves (AUC and incremental AUC) for RE, apoB-48 and triacylglycerol in the TRL fraction. No significant differences in these measurements were observed for the three meals. However, visual comparison of the postprandial responses to the three meals suggested that as meal MUFA content increased there was a tendency for the triacylglycerol, apoB-48 and RE responses to become biphasic as opposed to the typical monophasic response seen with the 12 % MUFA/17 % SFA meal. Comparison of the apoB-48 and RE responses for the three test meals confirmed other workers’ findings of delayed entry of RE relative to apoB-48 in TRL. The value of the two markers in investigating dietary fat absorption and metabolism is discussed.

Keywords

Fatty acidsRetinyl esterApolipoprotein B-48
Type
Short communication
Information
British Journal of Nutrition , Volume 81 , Issue 1 , January 1999 , pp. 51 - 58
Copyright
Copyright © The Nutrition Society 1999

References

Averna, MScip, RLMankowitz, K & Schonfeld, G (1993) Postprandial lipaemia in subjects with hypobetalipoproteinaemia and a single intestinal allelle for apo B-48 Journal of Lipid Research 34, 1957–1967.CrossRefGoogle Scholar
Berr, F & Kern, F Jr (1984) Plasma clearance of chylomicrons labeled with retinyl palmitate in healthy human subjects Journal of Lipid Research 25, 805–812.CrossRefGoogle ScholarPubMed
Brouwer, CB de Bruin, TWAJansen, H & Erkelens, DW (1993) Different clearance of intravenously administered olive oil and soybean oil emulsions: role of hepatic lipase American Journal of Clinical Nutrition 57, 533–539.CrossRefGoogle ScholarPubMed
Brown, MS & Goldstein, JL (1983) Lipoprotein metabolism in the macrophage: implications for cholesterol deposition in atherosclerosis Annual Reviews of Biochemistry 52, 223–261.CrossRefGoogle ScholarPubMed
Cohn, JSJohnson, EJMiller, JSCohn, SDMilne, RWMarcel, YLRussell, RM & Schaefer, EJ (1993) Contribution of apo B-48 and apo B-100 triglyceride-rich lipoproteins (TRL) to postprandial increases in the plasma concentration of TRL triglycerides and retinyl esters Journal of Lipid Research 34, 2033–2040.CrossRefGoogle ScholarPubMed
Cortner, JACoates, PM Le N-A Cryer, DRRagni, MCFaulkner, A & Langer, T (1987) Kinetics of chylomicron remnant clearance in normal and hyper-lipoproteinemic subjects Journal of Lipid Research 28, 195–206.CrossRefGoogle ScholarPubMed
de Bruin, TWABrouwer, CB van Linde-Sibenius Trip, MJansen, H & Erkelens, DW (1993) Different postprandial metabolism of olive oil and soybean oil: a possible mechanism of the high density lipoprotein conserving effect of olive oil American Journal of Clinical Nutrition 58, 447–483.CrossRefGoogle ScholarPubMed
Demacker, PNMReijnen, IGMKatan, MBStuyt, PMJ & Stahlenhoef, AFH (1991) Increased removal of remnants of triglyceride-rich lipoproteins on a diet rich in polyunsaturated fatty acids European Journal of Clinical Investigation 21, 197–203.CrossRefGoogle Scholar
Edelstein, C & Scanu, M (1986) Precautionary measures for collecting blood destined for lipoprotein isolation Methods in Enzymology 128, 151–155.CrossRefGoogle ScholarPubMed
Groot, PHE van Stiphout, WAHJKrauss, XHJansen, H van Tol, A van Ramshorst, EChin-On, SHofman, ACresswell, SR & Hanekas, L (1991) Postprandial lipoprotein metabolism of normolipidaemic men with and without coronary artery disease Arteriosclerosis and Thrombosis 11, 653–662.CrossRefGoogle Scholar
Grundy, SM & Mok, HYI (1976) Chylomicron clearance in normal and hyperlipidaemic man Metabolism, Clinical and Experimental 25, 1225–1291.CrossRefGoogle Scholar
Hazzard, WR & Bierman, EL (1976) Delayed clearance of chylomicron remnants following vitamin-A-containing oral fat loads in broad-β disease (Type III Hyperlipoproteinaemia) Metabolism, Clinical and Experimental 25, 777–801.CrossRefGoogle Scholar
Karpe, FBell, MBjorkegren, J & Hamsten, A (1995) Quantification of postprandial triglyceride-rich lipoproteins in healthy men by retinyl ester labelling and simultaneous measurement of apolipoprotein B-48 and B-100 Arteriosclerosis, Thrombosis and Vascular Biology 15, 199–207.CrossRefGoogle Scholar
Karpe, F & Hamsten, A (1995) Postprandial lipoprotein metabolism and atherosclerosis Current Opinions in Lipidology 6, 123–129.CrossRefGoogle ScholarPubMed
Keys, A (1970) Coronary artery disease in seven countries Circulation 41, I1–I211.Google Scholar
Kita, TGoldstein, JLBrown, MSWatanabe, YHornick, CA & Havel, RJ (1982) Hepatic uptake of chylomicron remnants in WHHL rabbits: a mechanism genetically distinct from the low density lipoprotein receptor Proceedings of the National Academy of Sciences USA 79, 3623–3627.CrossRefGoogle Scholar
Krasinski, SDCohn, JSRussell, M & Schaefer, EJ (1990 a) Postprandial plasma vitamin A metabolism in humans: a reassessment of the use of plasma retinyl esters as markers for intestinally derived chylomicrons and their remnants Metabolism 39, 357–365.CrossRefGoogle ScholarPubMed
Krasinski, SDCohn, JSSchaefer, EJ & Russell, RM (1990 b) Postprandial retinyl ester response is greater in older subjects compared with younger subjects Journal of Clinical Investigation 85, 883–892.CrossRefGoogle ScholarPubMed
Levy, ERoy, CCGoldstein, RBar-On, H & Ziv, E (1991) Metabolic fate of chylomicrons obtained from rats maintained on diets varying in fatty acid composition Journal of the American College of Nutrition 10, 69–78.CrossRefGoogle ScholarPubMed
Lovegrove, JIsherwood, SGJackson, KGWilliams, CM & Gould, BJ (1996) Quantification of apolipoprotein B-48 in triacylglycerol-rich lipoproteins by a specific enzyme-linked immunosorbent assay Biochimica et Biophysica Acta 1301, 221–229.CrossRefGoogle Scholar
Mahley, RW (1982) Atherogenic hyperlipoproteinemia. The cellular and molecular biology of plasma lipoproteins altered by dietary fat and cholesterol Medical Clinics of North America: Lipid Disorders 66, 375–402.Google Scholar
Matthews, JNSAltman, DGCampbell, MJ & Royston, P (1990) Analysis of serial measurements in medical research British Medical Journal 300, 230–235.CrossRefGoogle ScholarPubMed
Rassin, TLiron, MRubenstein, AArad, J & Weintraub, MS (1992) Vitamin A loading: an indicator of postprandial lipoprotein clearance in healthy and hypertriglyceridemic subjects Israeli Journal of Medical Science 28, 706–710.Google ScholarPubMed
Ross, AC & Zilversmit, DB (1977) Chylomicron remnant cholesteryl esters as the major constituent of very low density lipoproteins in plasma of cholesterol-fed rabbits Journal of Lipid Research 18, 169–181.CrossRefGoogle ScholarPubMed
Ruotolo, GZhang, HBentsianov, V & Le, N-A (1992) Protocol for the study of the metabolism of retinyl esters in plasma lipoproteins during postprandial lipaemia Journal of Lipid Research 33, 1541–1549.CrossRefGoogle Scholar
Sakr, SWHaourigui, MPaul, JLSoni, TVachter, D & Girard-Globa, A (1997) Fatty acid composition of an oral fat load affects chylomicron size in human subjects British Journal of Nutrition 77, 19–31.CrossRefGoogle ScholarPubMed
Schrezenmeir, JWeber, PProbst, RBiesalski, HKLuley, CPrellwitz, WKrause, U & Beyer, J (1992) Postprandial pattern of triglyceride-rich lipoprotein in normal-weight humans after an oral fat load: exaggerated triglycerides and altered insulin response in some subjects Annals of Nutrition and Metabolism 36, 186–196.CrossRefGoogle Scholar
Stender, S & Zilversmit, DB (1982) Comparison of cholesteryl ester transfer from chylomicrons and other plasma lipoproteins to aorta intima-media of cholesterol-fed rabbit Arteriosclerosis 2, 493–499.CrossRefGoogle Scholar
Weintraub, MSEisenberg, S & Breslow, JL (1987) Different patterns of postprandial lipoprotein metabolism in normal, Type IIa, TypeIII and TypeIV hyperlipoproteinemic individuals Journal of Clinical Investigation 79, 1110–1119.CrossRefGoogle Scholar
Weintraub, MSZechner, RBrown, AEisenberg, S & Breslow, JL (1988) Dietary polyunsaturated fats of the ?-6 and ?-3 series reduce postprandial lipoprotein levels: chronic and acute effects of fat saturation on postprandial lipoprotein metabolism Journal of Clinical Investigation 82, 1884–1893.CrossRefGoogle ScholarPubMed
Williams, CM (1997) Postprandial lipid metabolism: effects of dietary fatty acids Proceedings of the Nutrition Society 56, 679–692.CrossRefGoogle ScholarPubMed
Williams, CM (1998) Dietary interventions affecting chylomicron and chylomicron remnants Atherosclerosis (In the Press).CrossRefGoogle Scholar
Zampelas, APeel, AGould, BJWright, J & Williams, CM (1994) Polyunsaturated fatty acids of the n-6 and n-3 series: effects on postprandial lipid and apolipoprotein levels in healthy men European Journal of Clinical Nutrition 48, 88–96.Google ScholarPubMed
Zampelas, ARoche, HKnapper, JMEJackson, KGTornaritis, MHatzis, CGibney, MJKafatos, AGould, BJWright, J & Williams, CM (1998) Differences in postprandial lipaemic responses between northern and southern Europeans Atherosclerosis 139, 83–93.CrossRefGoogle ScholarPubMed