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Retinoid metabolism in the rat small intestine

Published online by Cambridge University Press:  08 March 2007

Simmy Thomas
Affiliation:
The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College, Vellore-632004, India
Ramamoorthy Prabhu
Affiliation:
The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College, Vellore-632004, India
Kunissery A. Balasubramanian*
Affiliation:
The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College, Vellore-632004, India
*
*Corresponding author: Dr K. A. Balasubramanian, fax +91 (416) 2232035, email wubalu@hotmail.com, wellcome@cmcvellore.ac.in
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Abstract

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Vitamin A (retinol) is essential for epithelial cell growth, differentiation and proliferation. The absorption of retinol occurs in the small intestine, and the metabolism of this vitamin is not well studied in this organ. The intestinal epithelium has a high rate of cell proliferation and differentiation, and the present study looked at the level of retinoids and metabolizing enzymes involved in their interconversion along the villus–crypt axis under normal conditions. Intestine was removed from control rats, and enterocytes at various stages of maturation and differentiation were quantified by the metal chelation method. Using HPLC, various retinoid concentrations in the cell homogenate and the metabolizing enzymes in the cytosol were quantified. The proliferating crypt cells were found to have a higher level of retinoic acid as well as of the enzymes involved in its formation, such as retinaldehyde oxidase and retinol dehydrogenase, compared with the villus cells, suggesting a possible role for this compound in intestinal epithelial cell proliferation and differentiation. The high level of retinol and high retinaldehyde reductase activity in the villus cells suggest the important role played by this enzyme in the conversion of dietary β-carotene to retinol via retinaldehyde. In summary, this study has given for the first time a detailed analysis of the retinoid levels and metabolizing enzymes in different cell populations in the rat small intestinal epithelium.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2005

References

Blaner, WS & Olson, JS (1999) The retinoids: biology, chemistry and medicine The Retinoids 2nd edn 229255 [Sporn, MB, Roberts, AB and Goodman, DS, editors] New York: Raven Press.Google Scholar
Chambon, P (1996) A decade of molecular biology of retinoic acid receptors. FASEB J 10, 940954.CrossRefGoogle ScholarPubMed
Chen, H, Namkung, MJ & Juchau, MR (1995) Biotransformation of all-trans-retinol and all-trans-retinal to all-trans-retinoic acid in rat conceptal homogenates. Biochem Pharmacol 50, 12571264.CrossRefGoogle ScholarPubMed
Deluca, HF & Roberts, AB (1969) Pathways of retinoic acid and retinol metabolism. Am J Clin Nutr 22, 945952.CrossRefGoogle ScholarPubMed
Eastwood, GL (1977) Gastrointestinal epithelial renewal. Gastroenterology 72, 962975.CrossRefGoogle ScholarPubMed
Giguere, V, Ong, ES, Segul, P & Evans, RM (1987) Identification of a receptor for the morphogen retinoic acid. Nature 330, 624629.CrossRefGoogle ScholarPubMed
Hanson, WR (1982) Proliferative and morphological adaptation of the intestine to experimental resection. Scand J Gastroenterol 74, (Suppl.) 1120.Google ScholarPubMed
Herr, FM, Wardlaw, SA, Kakkad, B, Albrecht, A, Quick, TC & Ong, DE (1993) Intestinal vitamin A metabolism: coordinate distribution of enzymes and CRBP (II). J Lipid Res 34, 15451554.CrossRefGoogle ScholarPubMed
Joseph, LW, Deborah, AS, Deborah, CR & Marc, SL (1997) Retinoic acid stimulates early cellular proliferation in the adapting remnant rat small intestine after partial resection. J Nutr 127, 12971303.Google Scholar
Lampen, F, Meyer, S, Arnhold, T & Nau, H (2000) Metabolism of vitamin A and its active metabolite All-trans-retinoic acid in small intestinal enterocytes. J Pharmacol Exp Ther 295, 979985.Google ScholarPubMed
Lintig, J & Vogt, K (2004) Vitamin A formation in animals: molecular identification and functional characterization of carotene cleaving enzymes. J Nutr 134, 251S – 256S.Google Scholar
Lotan, R (1996) Retinoids in cancer prevention. FASEB J 10, 10311039.CrossRefGoogle Scholar
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193, 265275.CrossRefGoogle ScholarPubMed
Moore, T (1930) Vitamin A and carotene. VI. The conversion of carotene to vitamin A in vivo. Biochem J 24, 692702.CrossRefGoogle ScholarPubMed
Napoli, JL (1999) Interactions of retinoid binding proteins and enzymes in retinoid metabolism. Biochim Biophys Acta 1440, 139162.CrossRefGoogle ScholarPubMed
Nau, H & Blanner, WJ (1999) Retinoids. The Biochemical and Molecular Basis of Vitamin A and Retinoid Action. New York: Springer.Google Scholar
Ong, DE (1993) Retinoid metabolism during intestinal absorption. J Nutr 123, 351355.CrossRefGoogle ScholarPubMed
Parlesak, A, Menzl, I, Feuchter, A, Bode, JC & Bode, C (2000) Inhibition of retinol oxidation by ethanol in the rat liver and colon. Gut 47, 825831.CrossRefGoogle ScholarPubMed
Petkovich, M (1992) Regulation of gene expression by vitamin A; the role of nuclear retinoic acid receptors. Annu Rev Nutr 12, 443471.CrossRefGoogle ScholarPubMed
Rexer, BN, Zheng, WL & Ong, DE (2001) Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells. Cancer Res 61, 70657070.Google ScholarPubMed
Thambidorai, D & Bachhawat, BK (1977) Purification and properties of brain alkaline phosphatase. J Neurochem 29, 503512.Google Scholar
Tomita, S, Tsujita, M & Ichikawa, Y (1993) Retinal oxidase is identical to aldehyde oxidase. FEBS Lett 336, 272274.CrossRefGoogle ScholarPubMed
Wald, G (1968) The molecular basis of visual excitation. Nature 219, 800807.CrossRefGoogle ScholarPubMed
Wang, JL, Swartz-Basile, DA, Rubin, DC & Levin, MS (1997) Retinoic acid stimulates early cellular proliferation in the adapting remnant rat small intestine after partial resection. J Nutr 127, 12971303.CrossRefGoogle ScholarPubMed
Weiser, MM (1973) Intestinal epithelial cell surface membrane glycoprotein synthesis. J Biol Chem 248, 25362541.CrossRefGoogle ScholarPubMed
Wojciech, A, Gonzalo, I & Regina, P (1999) Metabolism of retinaldehyde and other aldehydes in soluble extracts of human liver and kidney. J Biol Chem 274, 3336633373.Google Scholar
Wolf, G (1984) Multiple functions of vitamin A. Physiol Rev 64, 873937.CrossRefGoogle ScholarPubMed
Wong, MH, Stappenbeck, TS & Gordon, JI (1999) Living and commuting in intestinal crypts. Gastroenterology 116, 208215.CrossRefGoogle ScholarPubMed