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The effect of dietary n-3 fatty acids on serum concentrations of C-reactive protein: a dose–response study

Published online by Cambridge University Press:  01 August 2008

Trine Madsen*
Affiliation:
Department of Cardiology, Aalborg Hospital, Denmark
Jeppe H. Christensen
Affiliation:
Department of Nephrology, Aalborg Hospital, Denmark
Mogens Blom
Affiliation:
Department of Clinical Biochemistry, Hjørring/Brønderslev Hospital, Denmark
Erik B. Schmidt
Affiliation:
Department of Cardiology, Aalborg Hospital, Denmark
*
*Corresponding Author: Dr Trine Madsen, fax +45 99322361, email tmadsen@dadlnet.dk
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Abstract

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C-reactive protein (CRP) is a sensitive marker for low-grade inflammation. Long-chain n-3 polyunsaturated fatty acids (PUFA) have anti-inflammatory effects. The objective of the present study was to investigate the effect on serum levels of CRP of n-3 PUFA at two different doses. We also investigated correlations between CRP and the cellular contents of PUFA. Sixty healthy volunteers (twenty-five women and thirty-five men) were randomly assigned to three treatment groups in a double-blind design. The subjects received a supplement of either 6·6 g n-3 PUFA/d, 2·0 g n-3 PUFA/d or placebo (olive oil) for 12 weeks. CRP was measured using a highly sensitive assay. The median serum CRP concentration was 0·78 mg/l. No significant correlations were found between CRP and the content of n-3 PUFA in granulocytes or platelets. Subjects receiving n-3 PUFA had a significant (P<0·01) increase in the cellular contents of 20: 5n-3, 22: 5n-3 and 22: 6n-3, with the largest increase occurring in the group receiving 6·6 g PUFA/d. A significant (P<0·01) decrease in cellular content of 18: 2n-6 and 20: 4n-6 was observed simultaneously. Serum CRP concentrations, however, were unaffected by the PUFA-containing supplements. The present study shows that dietary supplementation with PUFA-containing supplements has no effect on serum concentrations of CRP, measured with a highly sensitive assay, in healthy subjects.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2003

References

Belluzzi, A, Boschi, S, Brignola, C, Munarini, A, Cariani, G & Miglio, F (2000) Polyunsaturated fatty acids and inflammatory bowel disease. American Journal of Clinical Nutrition 71, 339S342S.Google Scholar
Biasucci, LM, Liuzzo, G, Grillo, RL, Caligiuri, G, Rebuzzi, AG, Buffon, A, Summaria, F, Ginnetti, F, Fadda, G & Maseri, A (1999) Elevated levels of C-reactive protein at discharge in patients with unstable angina predict recurrent instability. Circulation 99, 855860.Google Scholar
Brown, AJ, Pang, E & Roberts, DC (1991) Persistent changes in the fatty acid composition of erythrocyte membranes after moderate intake of n-3 polyunsaturated fatty acids: study design implications. American Journal of Clinical Nutrition 54, 668673.Google Scholar
Calder, PC (2001) Polyunsaturated fatty acids, inflammation, and immunity. Lipids 36, 10071024.Google Scholar
Campos, SP & Baumann, H (1992) Insulin is a prominent modulator of the cytokine-stimulated expression of acute-phase plasma protein genes. Molecular and Cellular Biology 12, 17891797.Google Scholar
Castell, JV, Gomez-Lechon, MJ, David, M, Fabra, R, Trullenque, R & Heinrich, PC (1990) Acute-phase response of human hepatocytes: regulation of acute-phase protein synthesis by interleukin-6. Hepatology 12, 11791186.Google Scholar
Caughey, GE, Mantzioris, E, Gibson, RA, Cleland, LG & James, MJ (1996) The effect on human tumor necrosis factor alpha and interleukin 1 beta production of diets enriched in n-3 fatty acids from vegetable oil or fish oil. American Journal of Clinical Nutrition 63, 116122.Google Scholar
Chan, DC, Watts, GF, Barrett, PH, Beilin, LJ & Mori, TA (2002) Effect of atorvastatin and fish oil on plasma high-sensitivity C-reactive protein concentrations in individuals with visceral obesity. Clinical Chemistry 48, 877883.Google Scholar
Christensen, JH, Christensen, MS, Dyerberg, J & Schmidt, EB (1999) Heart rate variability and fatty acid content of blood cell membranes: a dose–response study with n-3 fatty acids. American Journal of Clinical Nutrition 70, 331337.Google Scholar
De Caterina, R, Liao, JK & Libby, P (2000) Fatty acid modulation of endothelial activation. American Journal of Clinical Nutrition 71, 213S223S.Google Scholar
Erlandsen, EJ & Randers, E (2000) Reference interval for serum C-reactive protein in healthy blood donors using the Da de Behring N Latex CRP mono assay. Scandinavian Journal of Clinical Laboratory Investigation 60, 3743.Google Scholar
Ernst, E, Saradeth, T & Achhammer, G (1991) n-3 Fatty acids and acute-phase proteins. European Journal of Clinical Investigation 21, 7782.Google Scholar
James, MJ & Cleland, LG (1997) Dietary n-3 fatty acids and therapy for rheumatoid arthritis. Seminars in Arthritis and Rheumatism 27, 8597.Google Scholar
James, MJ, Gibson, RA & Cleland, LG (2000) Dietary polyunsaturated fatty acids and inflammatory mediator production. American Journal of Clinical Nutrition 71, 343S348S.Google Scholar
Koenig, W, Sund, M, Frohlich, M, Fischer, HG, Lowel, H, Doring, A, Hutchinson, WL & Pepys, MB (1999) C-Reactive protein, a sensitive marker of inflammation, predicts future risk of coronary heart disease in initially healthy middle-aged men: results from the MONICA (Monitoring Trends and Determinants in Cardiovascular Disease) Augsburg Cohort Study, 1984 to 1992. Circulation 99, 237242.Google Scholar
Lee, TH & Austen, KF (1986) Arachidonic acid metabolism by the 5-lipoxygenase pathway, and the effects of alternative dietary fatty acids. Advances in Immunology 39, 145175.Google Scholar
Macy, EM, Hayes, TE & Tracy, RP (1997) Variability in the measurement of C-reactive protein in healthy subjects: implications for reference intervals and epidemiological applications. Clinical Chemistry 43, 5258.Google Scholar
Madsen, T, Skou, HA, Hansen, VE, Fog, L, Christensen, JH, Toft, E & Schmidt, EB (2001) C-reactive protein, dietary n-3 fatty acids, and the extent of coronary artery disease. American Journal of Cardiology 88, 11391142.Google Scholar
Meier-Ewert, HK, Ridker, PM, Rifai, N, Price, N, Dinges, DF & Mullington, JM (2001) Absence of diurnal variation of C-reactive protein concentrations in healthy human subjects. Clinical Chemistry 47, 426430.Google Scholar
Nielsen, GL, Faarvang, KL, Thomsen, BS, Teglbjaerg, KL, Jensen, LT, Hansen, TM, Lervang, HH, Schmidt, EB, Dyerberg, J & Ernst, E (1992) The effects of dietary supplementation with n-3 polyunsaturated fatty acids in patients with rheumatoid arthritis: a randomized, double blind trial. European Journal of Clinical Investigation 22, 687691.Google Scholar
Ridker, PM (2001) High-sensitivity C-reactive protein: potential adjunct for global risk assessment in the primary prevention of cardiovascular disease. Circulation 103, 18131818.Google Scholar
Ridker, PM, Cushman, M, Stampfer, MJ, Tracy, RP & Hennekens, CH (1997) Inflammation, aspirin, and the risk of cardiovascular disease in apparently healthy men. New England Journal of Medicine 336, 973979.Google Scholar
Rifai, N, Tracy, RP & Ridker, PM (1999) Clinical efficacy of an automated high-sensitivity C-reactive protein assay. Clinical Chemistry 45, 21362141.Google Scholar
Ross, R (1999) Atherosclerosis–an inflammatory disease. New England Journal of Medicine 340, 115126.Google Scholar
Schmidt, EB (1997) n-3 Fatty acids and the risk of coronary heart disease. Danish Medical Bulletin 44, 122.Google Scholar
Schmid, EB & Dyerberg, J (1989) n-3 Fatty acids and leucocytes. Journal of Internal Medicine 225, 151S158S.Google Scholar
Schmidt, EB, Pedersen, JO, Varming, K, Ernst, E, Jersild, C, Grunnet, N & Dyerberg, J (1991) n-3 Fatty acids and leukocyte chemotaxis. Effects in hyperlipidemia and dose–response studies in healthy men. Arterio-sclerosis and Thrombosis 11, 429435.Google Scholar
Simopoulos, AP (1999) Essential fatty acids in health and chronic disease. American Journal of Clinical Nutrition 70, 560S569S.Google Scholar
Steel, DM & Whitehead, AS (1994) The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein. Immunology Today 15, 8188.Google Scholar
Tataru, MC, Heinrich, J, Junker, R, Schulte, H, von Eckardstein, A, Assmann, G & Koehler, E (2000) C-reactive protein and the severity of atherosclerosis in myocardial infarction patients with stable angina pectoris. European Heart Journal 21, 10001008.Google Scholar
Toss, H, Lindahl, B, Siegbahn, A & Wallentin, L (1997) Prognostic influence of increased fibrinogen and C-reactive protein levels in unstable coronary artery disease. FRISC Study Group. Fragmin during Instability in Coronary Artery Disease. Circulation 96, 42044210.Google Scholar